10月21日 美国西北大学Prof. Cheng Sun 学术报告：Superresolution Spectroscopic Microscopy报告题目：Superresolution Spectroscopic Microscopy 附报告简介： Superresolution Spectroscopic Microscopy Cheng Sun Department of Mechanical Engineering, Northwestern University, Evanston IL 60208 The nanoscale architecture of biological systems underlies and regulates essentially all molecular machinery. Every cellular structure has a complex nanoscale organization ranging from individual macromolecules that are a few nanometers in size (e.g. protein, DNA) to macromolecular assemblies that may span tens of nanometers. The major scientific challenge is to understand these macromolecular assemblages, their function and interactions in non-perturbed, structurally and dynamically complex cellular systems. The importance of nanoscopic imaging is underscored by the 2014 Nobel Prize in Chemistry for the invention of optical nanoscopy. However, the potential of this technology can be immensely enhanced. In this talk, I will introduce spectroscopic photon localization microscopy, which is capable of simultaneously capturing the spatial distributions of photons emitted by individual molecules and their inherent spectroscopic signatures with sub-nm spectral resolution. As a result, the origins of photon emissions from different molecules can be identified according to their spectral differences. Using molecular identification, we can significantly extend the fundamental resolution limit of single molecule localization microscopy. Taking advantage of this unique capability, we demonstrated improvement in spatial resolution of PALM/STORM up to ten fold with selected fluorophores. |
学术报告 |
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中国 江苏省 南京市鼓楼区金银街15号
电话：+86 25 83592756 传真：+86 25 83595535
